Establishment and Application of Colloidal Gold Immune Chromatography Test Method for Detection of Canine Parvovirus Hemagglutination Inhibition Antibody Titer

The study was aimed to use colloidal gold immune chromatography technology to establish a rapid method for detection of canine serum canine parvovirus (CPV) hemagglutination inhibition (HI) titer and CPV vaccine immunization effect assessment.Double antibody sandwich method and monoclonal antibodies of anti-CPV hemagglutination antigen were used to prepare CPV antigen test strip.Canine serum with different proportion respectively was mixed with quantitative CPV antigen for full reaction,then dropped the mixture into the CPV colloidal gold test strip,so according to the highest serum dilution ratios when the test strip line T (line T) vanishes,it was to judge CPV antibodies in serum of the HI titer.This method had been used to detect 86 canine serum samples,at the same time,analyzing and comparing it with traditional hemagglutination inhibition test method.The results showed that the CPV antigen detection test strip was successfully prepared,and the reaction conditions and results of the test strip for detecting the titer of CPV-HI in canine serum were determined.The results indicated that when detecting CPV antigen after the dilution of different ratios of canine serum,the highest serum dilution ratios when the strip line T vanished and the HI titer had positive correlation.The highest dilution ratios of canine serum multiplied by 4 was the HI titer.The results of two methods had 90.7% consistency.This experiment established the colloidal gold immune chromatography test strip for the detection of CPV-HI titers method initially.This CPV-HI detection provided a simple and fast test method for the effect evaluation of CPV vaccine immune.     

金属阳离子夹心三核金咪唑基化合物的电子结构和光物理性质

采用混合密度泛函方法,对一系列包含金属离子(Cr2+、W2+、Fe2+、Ru2+、Cd2+、Cu+、Ag+、Au+、In+、Tl+、Li+、Na+)的咪唑基三核金夹心化合物基态分子结构进行了优化;并采用含时密度泛函方法对这些夹心化合物的垂直激发态进行研究,描述了与发光过程相关的前线分子轨道特征.计算结果表明:夹心金属离...     

动物源食品中氯霉素残留速测金标试纸条的研制

应用杂交瘤技术制备了抗氯霉素单克隆抗体,并根据竞争式胶体金免疫层析试验原理,研制了氯霉素检测免疫试纸条。研究结果表明,该单克隆抗体对氯霉素的亲合性好,特异性高;该金标试纸条适用于动物源食品中氯霉素残留的快速检测,对虾肉、蜂蜜样品的最低检出限为1.5μg/kg,对鲜奶的最低检出限为3μg/kg,检测时间只需5～8min。     

卡拉麦里山有蹄类野生动物自然保护区金矿生态恢复区的土壤理化性质和植被群落特征

[目的] 调查实施生态修复措施后的矿区土壤状况与植物群落的恢复情况,为矿区修复治理措施提供评价依据。[方法] 以新疆卡拉麦里山有蹄类野生动物自然保护区的金矿生态恢复区为研究对象,通过样方法和室内试验,进行研究区植被特征、植物群落多样性调查和土壤理化性质检测分析。[结果] 与原生对照区相比,经过生态修复工作的金矿生态修复区土壤pH值、电导率、有机质、总氮、全钾的含量普遍较低;植物物种较少,以人为撒播草种的藜科植物占优势;植物群落多样性指数低,与原生对照联系紧密;植物群落主要受土壤有机质、有效磷、速效钾等含量的影响。[结论] 在进行生态修复措施后,青河大沙沟和富蕴大沙沟的土壤理化性质和植物群落改善较明显,恢复效果显著。金矿恢复区由于恢复时间较短,所以植物种类稀少,群落稳定性低,土壤理化性质的含量普遍不高,还需要时间进行恢复。     

Development of Colloidal Gold Rapid Detection Method of β-lactams and Tetracyclines in Milk

Colloidal gold immunochromatographic was uesd for the determination of β-lactams and tetracyclines in milk. Tetracyclines hapten was obtained by chemical synthesis. Immunogen and envelope antigen were prepared by cephalosporins and tetracyclines hapten coupled to carrier protein. Monoclonal antibodies were obtained by immunogen immunizing mice. A rapid and simple colloidal gold immunochromatographic assay was established for the determination of β-lactams and tetracyclines in milk. 12 kinds of β-lactams and 4 kinds of tetracyclines were detected at the same time by the strip. The milk sample could be used to be detected without dilution. The detection time could be 3 to 5 min. The method could be efficiently used for mass samples screening in primary laboratory.     

基于GARCH模型的黄金指数与美元指数波动性研究

近几年的黄金市场与美元指数市场波动都比较大,但波动的方向不一致。通过对两者的波动进行研究,主要有单位根检验、ARCH效应的检验、GARCH模型分析以及因果关系检验,结果表明,黄金指数GARCH(1,1)最适合描述其市场波动,美元指数GARCH（2,1）最适合描述其市场波动,美元指数的预测对黄金指数的预测会有帮助。     

食品中霉菌毒素检测技术研究新进展

介绍了生物芯片、生物传感器、胶体金技术、EILSA和免疫荧光法在霉菌毒素检测中的研究进展,提出要加强霉菌毒素的检测技术,需不断引入新技术和新方法。     

猪伪狂犬病血清抗体两种检测方法的比较

应用酶联免疫吸附试验和免疫金标检测试纸两种方法，同时对64头份猪血清样品进行猪伪狂犬病血清抗体检测，试验结果显示：两种方法检测的64头份猪血清中伪狂犬病抗体阳性率均为62．5％(40／64)，符合率达到100％。两种方法都具有微量、特异、准确的优点。前者需要用酶标仪等仪器，试验时间相对较长，成本高，但能用准确的数字表达抗体水平；后者不需要任何仪器设备，试验时间短，成本低，适用于基层兽医站、养殖场使用，以及大面积开展猪伪狂犬病抗体检测的使用。     

犬瘟热病毒单克隆抗体的研制及胶体金试纸条检测方法的建立

本研究利用原核表达、纯化的犬瘟热病毒(Canine distemper virus,CDV)N 蛋白免疫BALB/C小鼠,成功筛选到两株分泌CDV N 蛋白单克隆抗体的杂交瘤细胞株,分别命名为D3、D6。2株杂交瘤细胞分泌单克隆抗体敏感性、特异性良好,能识别不同来源的犬瘟热病毒株,单抗亚类均为IgG1。以D3作为金标抗体,D6作为检测抗体,兔抗鼠IgG作为质控线抗体,制备犬瘟热病毒胶体金检测试纸条,检测犬瘟热病毒敏感性为1000TCID50,能有效检测区分犬瘟热病毒、犬细小病毒(Canine parvovirus,CPV)、犬腺病毒2型(Canine adenovirus type 2,CAV2)、狂犬病病毒(Rabies virus,RV)。综上,本研究建立的胶体金试纸条检测方法,灵敏度高、特异性强,适用于犬瘟热病毒临床快速诊断。